The 325th Airborne Infantry Regiment is a light infantry parachute insertion fighting force of the United States Army. The subordinate units of the regiment constitute the bulk of the infantry elements assigned to the 2nd Infantry Brigade Combat Team, 82nd Airborne Division.

The 325th Airborne Infantry Regiment deploys anywhere in the world, within 18 hours of notification. The regiment conducts forced entry parachute assaults to seize, retain and defend airfields or other assets, then increases combat power in order to control land, people, and resources

Called to active duty again during World War II on 25 March 1942, the regiment formed under the command of Colonel Claudius M. Easley at Camp Claiborne, Louisiana. After initial training, the regiment was reorganized as a glider infantry unit when the 82nd Infantry Division was reorganized as the 82nd Airborne Division in August 1942, and the 325th was redesignated the 325th Glider Infantry Regiment.

After further training, the regiment deployed to North Africa under the command of Colonel Harry L. Lewis. The regiment was not committed to combat until they conducted a landing at Salerno, Italy, on 15 September 1943. It was not, however, by glider but by landing craft

After service in the Italian Campaign, the regiment deployed to England and conducted further training in preparation for Operation Overlord. Conducting a glider-borne assault on 7 June 1944, the day after the initial Normandy landings, the regiment fought in Normandy for over a month, sustaining heavy casualties before being withdrawn to England. On 9 June 1944 the 325th earned its first and onlyMedal of Honor of the war, belonging to Private First Class Charles N. DeGlopper.

Committed to another glider-borne assault in September 1944, the regiment fought in Holland as part of Operation Market Garden and later saw service in the Battle of the Bulge.